| Lecture No. | Topic |
| 1 & 2 | Scope and importance of biochemistry. Buffer system and pH. Dissociation of and Henderson – Hasselbalch equation |
| 3 & 4 | Structure of cell membranes and transport across cell membranes. Donnan membrane equilibrium |
| Biochemistry of carbohydrates: Biological significance (structure and properties) of: | |
| 5 | Monosaccharides (ribose, glucose, fructose, galactose, mannose and amino sugars) |
| 6 | Disaccharides (maltose, isomaltose, lactose, sucrose and cellobiose |
| 7 | Polysaccharides (starch, dextrins, dextrans, glycogen, cellulose, inulin, chitin) |
| 8 | Mucopolysaccharides including bacterial cell wall polysaccharides |
| Biochemistry of lipids: | |
| 9 & 10 | Properties (structure) and biological significance of simple, compound and derived lipids and lipoproteins |
| 11 | Structure and functions of prostaglandins. Chemistry of bile and bile salts |
| 12 | Amino acids: Classification and structure of neutral basic and acidic amino acids |
| 13 | Properties of amino acids: amphoteric nature, optical activity and peptide bond formation |
| 14 | Structure properties, biological significance and chemical reactions of proteins |
| Biochemistry of nucleic acids | |
| 15 | Chemistry of purines and pyrimidines, nucleosides and nucleotides. Biological significance of nucleosides and nucleotides |
| 16 | Structures and functions of deoxyribonucleic acid (DNA) and a typical ribonucleic acid |
| Lecture No. | Topic |
| 1 & 2 | Enzymes: Definition, classification and EC numbering of enzymes. Coenzymes, cofactors &isoenzymes. Enzyme units: International units, katal, turnover number & specific activity. |
| 3 & 4 | Properties: Protein nature, Enzyme-substrate complex formation, Modern concept of enzyme specificity of enzyme action: substrate specificity, Group specificity, stereo specificity, optical specificity. |
| 5 | Factors influencing enzyme action: Effects of temperature, pH, concentration of substrate and enzyme; Enzyme inhibition: Competitive, non competitive, uncompetitive and suicidal inhibition allosteric enzymes. |
| 6 & 7 | Biological Oxidation: Enzymes and coenzyrnes involved in oxidation and reduction viz. Oxidoreductase, Oxidases, oxygenases, dehydrogenases, Hydroperoxidases& cytochromes. |
| 8 & 9 | Respiratory chain / electron transport chain, oxidative phosphorylation, inhibitors, uncouplers and other factors influencing electron transport chain |
| Carbohydrate metabolism along with bioenergetics: | |
| 10 | Glycolysis |
| 11 | Kreb’s cycle along with energetics |
| 12 | Glyoxilate cycle, HIVIP shunt. Gluconeogenesis |
| 13 | Cori cycle, Glycogenesis, Glycogenolysis |
| 14 | Hormonal control of carbohydrate metabolism & regulation of blood sugar. |
| Lipid metabolism with bioenergetics: | |
| 15 | Beta oxidation of fatty acid, Ketone body formation |
| 16 | Biosynthesis of fatty acids. Triacylglycerol metabolism |
| 17 | Phospholipids & lipoprotein metabolism |
| Protein metabolism: | |
| 18 | Biosynthesis of proteins |
| 19 | Degradation of protein: deamination, transamination & decarboxylation of amino acids |
| 20 | Ammonia transport and urea cycle. |
| 21 & 22 | Nucleic acids: Metabolism of purines and pyrimidines |
| 23 | DNA & RNA biosynthesis |
| 24 | Integration of metabolism |
| 25 | Metabolic functions of macro nutrients |
| 26 | Metabolic functions of micro nutrients |
| Metabolic functions of lipid soluble vitamins: | |
| 27 | Metabolic functions of Vitamin A & E |
| 28 | Metabolic functions of Vitamin D & K |
| Metabolic functions of water soluble vitamins: | |
| 29 | Vitamin C |
| 30 | Thiamine, Riboflavin, Niacin & Pyridoxine, |
| 31 | Pantothenic acid, Folic acid & Cynocobalamin. |
| 32 | Use of isotope in metabolic studies. |
| Lecture No. | Topic |
| Endocrinology | |
| 1 | Definitions, basic concepts and scope of animal biotechnology. |
| 2 & 3 | Recombinant DNA technology: Principles, procedures and applications |
| 4 & 5 (Micro) |
Gene cloning, vectors and expression vectors. New generation vaccines: subunit, recombinant and recombinant vectored vaccines |
| 6 | Animal tissue culture: Principles, procedure and application |
| 7 & 8 | Transformation and cell lines: Principles, procedure and application |
| 9 | Polymerase chain reaction (PCR) and its application in molecular diagnosis. DNA probes, nucleic acid hybridization |
| 10 & 11 | Transfection, Transgenic animal and biopharming |
| 12 & 13 | Construction of genomic library and cDNA library |
| 14 & 15 | DNA sequencing – genome, sequencing and mapping |
| 16 | Marker assisted selection and gene banking |
| 17 | Nutritional biotechnology including bioconversion of lignocellulose, genetic manipulation of microbes for improved feed utilization and health |
| 18 & 19 | Principles of transfer of nucleic acids and proteins (Southern, Northern and Western blotting), DNA fingerprinting and its applications |
| 20 | Tumour markers and acute phase proteins |
| 21 | Hybridoma and monoclonal antibodies |
| 22 | Fermentation process |
| 23 | Technologies for milk, meat and leather |
| 24 (Gyn) | Biotechnological application in animal improvements: Embryo biotechniques, in vivo embryo production, Embryo micromanipulation |
| 25 | In vitro embryo production, Embryo sexing |
| 26 | Embryo preservation, Embryo cloning |
| 27 & 28 | Ethics and regulatory issues in biotechnology, IPR, Bioinforrnatics |